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KMID : 0545120010110030428
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Journal of Microbiology and Biotechnology
2001 Volume.11 No. 3 p.428 ~ p.434
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Levan-Producing Bacillus subtilis BS 62 and Its Phylogeny Based on Its 16S rDNA Sequence
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Choi, Seong Hyun
Sung Chang/Choi, Woo Young
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Abstract
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A viscous substance producer strain BS 62, which was isolated from conventional Chungkookjang, was examined for its productivity of levansucrase and levan during soybean fermentation at 37℃. After one day of cultivation, the enzyme activity reached the highest level, 8units ㎖^-1. Extracts of fermented soybeans were precipitated by ethanol and hydrolyzed by either 0.1N HC1 or invertase, and the hydrolyzates were analyzed using thin layer and ion chromatographies. Fructose was the only sugar detected. This suggests that fructose was derived from the levan produced by the strain BS 62 during soybean fermentation. The aerobic, endospore-forming bacterium BS 62 was identified as a Bacillus subtilis sp., based on the composition of its cellular fatty acids and phylogeny, which was determined by its 16S rDNA sequence.
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